Comparison of the capacitation-like state of cooled boar spermatozoa with true capacitation
نویسندگان
چکیده
منابع مشابه
Effect of processing of semen on capacitation time of fresh and frozen-thawed boar spermatozoa.
Ovulation time of gilts was controlled by oral treatment with altrenogest (17-allyl-hydroxyestra-4,9,11-trien-3-one) followed by gonadotropic hormone injections. Gilts were laparotomized 42 to 44 h after injection of human chorionic gonadotropin. Spermatozoa were surgically placed into ligated oviducts of gilts after one of the following semen treatments: frozen and thawed in Beltsville thawing...
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Polyclonal avian antibody was used partially to characterize the pig sperm lactadherin P47. P47 is a mosaic protein, composed of two epidermal growth factor (EGF)-like domains and two C1/C2 domains. P47 is homologous to the bovine mammary gland protein MGP 53/57 and mouse milk fat globule protein. Expression of P47 along the male genital tract and its localization on spermatozoa during post-tes...
متن کاملCharacterization of the endocannabinoid system in boar spermatozoa and implications for sperm capacitation and acrosome reaction.
Anandamide (AEA) is the endogenous ligand of cannabinoid (CB) receptors, and as such it plays several central and peripheral activities. Regulation of female fertility by AEA has attracted growing interest, yet a role for this endocannabinoid in controlling sperm function and male fertility in mammals has been scarcely investigated. In this study we report unprecedented evidence that boar sperm...
متن کاملAnalysis of chaperone proteins associated with human spermatozoa during capacitation.
Mammalian spermatozoa must undergo a post-ejaculatory period of maturation, known as capacitation, before they can engage in the process of fertilization. Studies in the mouse have established that capacitation facilitates sperm-zona recognition via mechanisms that involve the appearance of tyrosine phosphorylated chaperone proteins on the sperm surface overlying the acrosome, the site of sperm...
متن کاملCat (Felis catus L.) spermatozoa require capacitation.
In the oestrous female cat, fertilization can take place when spermatozoa are introduced into the vagina up to 49 hr after an ovulation-inducing injection of human chorionic gonadotrophin. The spermatozoa require residence in the female reproductive tract before they are capable of fertilizing the eggs in vitro. No eggs were fertilized by freshly ejaculated spermatozoa. One of four oestrous fem...
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ژورنال
عنوان ژورنال: Reproduction
سال: 2001
ISSN: 1470-1626,1741-7899
DOI: 10.1530/rep.0.1220889